川藏地区牦牛牛病毒性腹泻病毒分子流行病学调查及分离鉴定

doi:10.11843/j.issn.0366-6964.2018.03.018

Abstract

Abstract:

This study was conducted to investigate the infection status of Bovine viral diarrhea virus (BVDV) and the main epidemic subtypes and genetic variation of BVDV in the Sichuan-Tibet Plateau region. A total of 149 clinical diarrhea yak fecal samples were collected from Sichuan province and Tibet region in 2016, and the genetic variation of BVDV in yak was studied according to the variation of 5'-UTR, Npro and E2. Meanwhile, the BVDV strain of yak was isolated and identified by RT-PCR, cell isolation and indirect immunofluorescence. The results showed that the positive rate of BVDV was 19.46% (95% confidence interval (CI)=13.4% -26.7%) in 149 samples of clinical diarrhea yak feces. The positive rate of BVDV in yak in Tibet region was 27.14% (95% CI=17.2% -39.1%) and 12.66% (95% CI=6.2%-22.0%) in Sichuan province. The phylogenetic tree was established based on the sequences of 5'-UTR, Npro and E2. The results showed that 10 samples belongs to BVDV-1, BVDV-1a subtype (n=4) and 1d (n=6). In addition, two strains of cytopathic (cp) BVDV were identified and identified as subgroups of BVDV-1a and 1d, named SWU-Z10 and SWU-L8, respectively. The results showed that BVDV infection is present in yak in the Sichuan-Tibet Plateau region, and BVDV-1a and 1d are the dominant subtype of yak infection in Sichuan-Tibet Plateau region. The results of the study provide basic data for the comprehensive prevention and control of yak diarrhea in Sichuan-Tibet Plateau region. The data obtained enrich the molecular epidemiological investigation data of BVDV, and provide the theoretical basis for the development of BVDV vaccine for yak.

CLC Number:

S852.659.6

CHEN Xin-nuo, XIAO Min, RUAN Wen-qiang, QIN Si-nan, YUE Hua, TANG Cheng, ZHANG Bin. Molecular Epidemiological Investigation and Isolation of Bovine Viral Diarrhea Virus in Yak in Sichuan-Tibet Plateau Region[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 606-613.

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Molecular Epidemiological Investigation and Isolation of Bovine Viral Diarrhea Virus in Yak in Sichuan-Tibet Plateau Region

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